School Science Lessons
2017-075 SP MF LI
Please send comments to: J.Elfick@uq.edu.au

18.0.0 Environmental chemistry, pollution
Table of contents

18.6.0 Air pollution

12.13.0 Hardness in water

18.1.0 pH tests

18.7.0 Water pollution

18.8.0 Tests for water pollution

Water testing chemicals, Commercial


18.6.0 Air pollution
12.6.0.1 Acid rain, SOx, burning sulfur, compound
3.48 Acid rain and nitrogen oxides, NOx
18.6.2 Air pollution from burning refuse
37.42.1 Atmosphere & greenhouse gases
3.39.0 Carbon monoxide
18.6.0.1 Comm air pollutants, safe air and clean air
18.6.0.2 Indoor air pollution, formaldehyde
37.43.1 Global warming and climate change

Experiments
31.4.44 Cottrell smoke precipitator
18.6.3 Danger of vehicle exhausts, tailpipe gases
16.7.28 Harmful substances in cigarette smoke
18.6.2.1 Tests for air pollution from burning refuse
18.6.3.1 Tests for carbon monoxide with a gas detector

18.1.0 pH Tests
pH meters, "Scientrific", (Commercial)
18.1.0 pH tests
18.1.0.1 Acidity and alkalinity
6.17.2 Soil acidity, pH:
18.1.1 Tests for pH with universal indicator
18.1.2 Tests for pH of water in the laboratory
18.1.3 Tests for pH of rainwater
18.1.4 Tests for pH of standing water

18.2.0 Tests for hardness in water
18.2.6.1 Tests for electrical conductivity with a conductivity meter
18.2.4 Tests for contamination of groundwater from refuse deposits
18.2.3 Tests for extracted soluble solids from rainwater
18.2.1 Tests for insoluble solids in rainwater
18.2.2.3 Tests for sulfates in groundwater
18.2.0 Total dissolved solids and suspended solids in water, Beer-Lambert law

18.7.0 Water pollution
Water quality "Scientrific", (commercial website)
24.0.0 Water, (Chemistry), (Experiments)
18.7.0 Bottled Water
18.2.7 Cations and anions in rain, rivers and sea water
18.2.6 Conductivity, TDS and electrical conductivity
18.2.2.1 Tests for chlorides in groundwater
35.23.9 Coal seam gas, CSG, and coal to liquid, CTL, projects
18.6.6 Colour of water
18.6.6.1 Tests for colour of water
18.6.9 Colorimeter tests, test methods
18.6.1 Tests for contamination of groundwater by refuse, hazardous wastes
12.12.05 Detergent phosphates
18.7.1 Drinking water test media
18.7.2 Effect of household detergents on freshwater organisms
10.5.7 Gases dissolved in a water sample
18.6.7 Hydrogen ion concentration of water
18.5.1 Tests for characteristics of river, lake or ocean
16 Tests for turbidity
19 Total solids
18.6.7.1 Tests for hydrogen ion concentration of water
18.4.0 Tests for ions in a water sample
3.3.5 Mercury's silent toll on the world's wildlife
4.3.11 Micro-organisms and water pollution
35.23.10 Oil shale and fracking, (hydraulic fracturing), (Geology)
18.2.2.2 Iron in drinking water
5.41 Keep water clean (Primary)
Nessler's reagent, Tests for ammonium radical, NH4+, free and combined ammonia
18.2.5 Salinity
18.6.5 Smell of water, hydrogen sulfide
18.6.5.1 Tests for smell of water
18.6.8 Temperature of water
18.6.8.1 Tests for temperature of water
18.3.0a Tests for air and dissolved oxygen in water
18.5.0 Tests for standing water
18.6.4 Water tests

18.3.0 Gases dissolved in a water sample
Water quality, Dissolved oxygen, "Scientrific", (commercial website)
10.5.7 Gases dissolved in a water sample
18.3.3 Tests for dissolved oxygen, titration
18.3.2 Oxygen content of water, dissolved oxygen, DO (Winkler method)
18.3.0a Tests for air and dissolved oxygen in water
18.3.1 Tests for air dissolved in a water sample

18.5.0 Tests for standing water, (Environmental chemistry)
18.5.2 Test for anions in sewage and tap water
18.5.1a Eutrophication of waterways
18.5.3 Tests for water samples
18.5.1 Tests of river, lake or ocean

18.8.0 Tests for water pollution, (Environmental chemistry)
18.3.0a Tests for air and dissolved oxygen in water
18.3.1 Tests for air, dissolved in a water sample
18.5.2 Test for anions in sewage and tap water
18.5.1 Tests for characteristics of river, lake or ocean
18.2.2.1 Tests for chlorides in groundwater
18.6.6.1 Tests for colour of water
18.6.1 Tests for contamination of groundwater by refuse, hazardous wastes
18.3.3 Tests for dissolved oxygen, titration
18.6.7.1 Tests for hydrogen ion concentration of water
18.4.0 Tests for ions in a water sample
18.4.1 Tests for phosphate ions in water
18.5.1 Tests of river, lake or ocean
18.6.5.1 Tests for smell of water
18.6.8.1 Tests for temperature of water
18.5.0 Tests for standing water
16.0 Tests for turbidity

18.1.0 pH tests
See 12.10.7.0: Buffer solutions
| 3.9.0 Solubility and solutions
pH tests use an indicator that changes colour with changes in the concentration of hydrogen ions, or the acidity of the solution.
The pH value of the ocean changes very little when acids or alkalis are added or when diluted with water because oceans are buffered
solutions.
However, many rivers and lakes are weakly buffered so their pH may change rapidly if acids or bases are added.
The oceans, and even some rivers and lakes, contain many different equilibrium reactions.
For example, when carbon dioxide dissolves in the ocean all the following reaction can occur to produce about pH 8 that remains
constant because of the "carbonate buffer".
The pH of drinking water normally ranges from 5.5 to 9.0. At pH levels of less than 7.0, corrosion of water pipes may occur, releasing
metals into the drinking water.
This is undesirable and can cause other concerns if concentrations of such metals exceed recommended limits.
The reactions below produce both H+ and OH- ions, but [OH-] > [ H+] so the pH value remains steady at about pH 8.
Phosphates and silicates have chemical reactions that contribute to buffering capacity.
CO2 (g) <--> CO2 (aq)
H2O + CO2 (aq) <--> H2CO3
H2CO3 <--> H+ + HCO3-
HCO3- <---> H+ + CO32-
HCO3- <---> CO2 (aq) + OH-
CO32- + H2O <---> HCO3- + OH-

18.1.0.1 Acidity and alkalinity
Instead of listing all the reactions that contribute to pH of oceans and rivers, you can call the capacity of chemical species together in
the water to neutralize a strong acid acidity or alkalinity, as measured in a pH value of hydrogen ion concentration.
pH log10(1 / [H+]). For many lakes and rivers, if the water has values between pH 5.0 and pH 8.0, the water is often sufficiently
buffered so that, if acids, bases or salts are added, the pH value does not change greatly.
Some salts, e.g. ammonium sulfate, ammonium chloride and aluminium chloride, and some gases, e.g. carbon dioxide and sulfur
dioxide, will increase the acidity of water.
A low alkalinity river or lake may have sudden pH value changes if acid or acid industrial waste pollute the water.
A sudden change in pH value, below pH 5.0 or above pH 8.0, kills many organisms, including fish.
Alkalinity is a measure of the presence of bicarbonate, carbonate or hydroxide constituents.
Concentrations less than 100 ppm are desirable for domestic water supplies. The recommended range for
drinking water is 30 to 400 ppm.
A minimum level of alkalinity is desirable because it is considered a buffer that prevents large variations in pH.
Alkalinity is not detrimental to humans.
Moderately alkaline water (less than 350 mg / l) in combination with hardness, forms a layer of calcium or magnesium carbonate that
tends to inhibit corrosion of metal piping.
Many public water utilities employ this practice to reduce pipe corrosion and to increase the useful life of the water distribution system.
High alkalinity (above 500 mg / l) is usually associated with high pH values, hardness and high dissolved solids and has adverse effects
on plumbing systems, especially on hot water systems where excessive scale reduces the transfer of heat to the water.
Water with low alkalinity, < 75 mg / l).
For example, some surface waters and rainfall, is subject to changes in pH because of dissolved gasses that may be corrosive to
metallic fittings

18.1.1 Tests for pH with universal indicator
Use Universal Indicator with a constant technique.
Construct a database of the pH of water at the same places, e.g. along a river bank, but at different times.
Collect many readings and look for patterns.

18.1.2 Tests for pH of water in the laboratory
See 3.34.3: Carbon dioxide, Solubility of acidic oxide carbon dioxide in water, acidity of soda water.
Use Universal Indicator to test the pH of deionized water or demineralized water, tap water, boiled tap water, tank water, bottled
water, non-gaseous mineral water, gaseous mineral water, soda water, fizzy lemonade.

18.1.3 Tests for pH of rainwater
Collect rainwater in a clean container.
Use Universal Indicator to test the pH of an isolated rain shower (a "rain incident") the first rain after a dry period, during continued
periods of rain (a "rain episode") during different wind directions, In an urban area, in a rural area, in an area near an industrial plant,
e.g. a powerhouse or steel works.
Rain water is in equilibrium with the carbon dioxide in the atmosphere that forms carbonic acid, a weak acid (ka = 1.75 × 10-5),
the solution under normal atmospheric conditions has pH 5.7.
The average pH of rain is pH 5.0, with range of pH 5.6 to pH 4.5, but in eastern industrialized North America the average pH of
rainwater is reported as pH 4.2.

18.1.4 Tests for pH of standing water
1. Use Universal Indicator to test the pH of temporary standing waters, puddles, tree boles, along rivers, shallow and deep water,
main stream and tributary, lake or sea or ocean, including shallow and deep water, sewage water before and after treatment, rivers,
where they discharge sewage water, effluent discharged into rivers from factories and industrial plants.
2. Dip the pH meter into solution.
Stir gently for a few seconds, until the readings stabilize.
Record reading.
Rinse pH detector tip with deionized water.

18.2.0 Total dissolved solids and suspended solids in water, Beer-Lambert law
Water quality, Colorimeter, Beer's Law experiments, "Scientrific" (Commercial)
Colorimetric tests based on Beer's Law (Beer-Lambert law) are based on the observation that the higher the concentration of a
substance in a sample, the darker the colour developed in the test, i.e. more light is absorbed by the sample.
So the intensity of light (electromagnetic radiation) that passes through a sample diminishes exponentially with the concentration and
thickness of the sample.
Absorption of light depends on the pathway length and concentration of the material passed through.
The total dissolved solids test measures the total amount of dissolved minerals in water.
The solids can be iron, chlorides, sulfates, calcium or other minerals found on the surface of the earth.
The dissolved minerals can produce an unpleasant taste or appearance and can contribute to scale deposits on pipe walls.
The following levels of total dissolved solids are expressed in mg / Litre:
Less than 500 Satisfactory,
500 to 1 000 Less than desirable,
1 000 to 1 500 Undesirable,
Over 1 500 Unsatisfactory.
The only effective means of reducing total dissolved solids is by using reverse osmosis; however, removal is not economical.

18.2.1 Tests for insoluble solids in rainwater
Use a previously weighed filter paper kept in a desiccator.
Collect the water in a very clean beaker.
Swirl the water sample to keep the dirt suspended and filter 100 mL into a measuring cylinder.
Note the volume of the filtrate.
Dry the filter paper in the dissector.
Weigh the filter paper and insoluble particles.

18.2.2.1 Tests for chlorides in groundwater
The chlorides in groundwater can occur naturally or be caused by pollution from sea water or industrial wastes.
Chloride concentration above 250 mg / Litre can produce a distinct taste in drinking water.
Where chloride content is known to be low, a noticeable increase in chloride concentrations may indicate pollution from sewage sources.
The following levels of chlorides are expressed in mg / Litre:
0 to 250 Acceptable,
250 to 500 Less than desirable,
500 to 1 000 Undesirable,
Over 1 000 Unsatisfactory.

18.2.2.2 Iron in drinking water
The iron in drinking water can give a rusty colour to laundered clothes and may affect taste.
Frequently found in water because of large deposits in the surface of the earth.
Iron can also be introduced into drinking water from iron pipes in the water distribution system.
In the presence of hydrogen sulfide, iron causes a sediment to form that may give the water a blackish colour.
Maximum concentration for iron in drinking water of 1.0 mg /Litre.
The following levels of iron (Fe) are expressed in mg / Litre:
0 to 0.3 Acceptable,
0.3 to 1.0 Satisfactory (however, may cause staining and objectionable taste)
Over 1.0 Unsatisfactory.
Iron as it exists in natural groundwater is in the soluble (ferrous) state but, when exposed to oxygen, is converted into the insoluble
(ferric) state with its characteristic reddish brown or rusty colour.
If allowed to stand long enough, this rusty sediment will usually settle to the bottom of a container.
However, it is difficult to use this type of settling to remove the iron.
The four options for removing iron from potable water are as follows:
1. For dissolved iron in concentrations up to 2.0 mg / litre, add food grade phosphate that sequesters the dissolved iron, i.e. keeps the
iron in solution.
2. Zeolite softening can remove up to 10 mg / litre of dissolved iron.
3. Potassium permanganate can remove up to 10 mg / litre of iron and will remove dissolved as well as particulate iron.
The permanganate provides oxygen to oxidize and precipitate any dissolved iron.
4. Liquid chlorine solution can be used for any quantity of iron, dissolved or not, and kill iron bacteria.

18.2.2.3 Tests for sulfates in groundwater
The sulfates in groundwater are caused by natural deposits of magnesium sulfate, calcium sulfate or sodium sulfate.
Concentrations should be below 250 ppm.
Higher concentrations cause laxative effects.
Sulfates cannot be economically removed from drinking water.
The following levels of sulfates are expressed in mg / l:
1. 0 to 250 Acceptable, 2. 250 to 500 Can be tolerated, 3. 500 to 1 000 Undesirable, 4. Over 1 000 Unsatisfactory.

Experiment
Weigh a clean dry evaporating dish.
Put the filtered rainwater in the evaporating dish and heat to dryness.
If heated too rapidly, the solution "spits" and some solids may be lost.
When you evaporate the solution to dryness, cool the evaporating dish and leave it in a desiccator for one day.
Record the weight of the dissolved solids.

18.2.3 Tests for extracted soluble solids from rainwater
Observe the dried filtrate under a microscope to identify its origin, e.g. sand, soot, organic matter or coal washing residues.

18.2.4 Tests for contamination of groundwater from refuse deposits
The main problem of waste disposal today is the possible contamination of groundwater by direct seepage from refuse deposits or by
substances leached from such deposits.

Experiments
1. Support a funnel with a top diameter of 100 mm with a support stand using a right angle clamp and a universal clamp.
Put a small wad of cotton wool in the funnel and then fill it with soil to within a thumb width of the top.
Put a beaker beneath it.
Spread about 1 g of copper (II) sulfate on the soil and pour water over it.
The water dissolves the copper (II) sulfate and seeps into the soil.
After a few minutes the blue copper (II) sulfate solution drips from the funnel into the beaker placed beneath it.

2. Spread an equal amount of sodium sulfate, potassium sulfate or ammonium sulfate on the soil in the funnel, instead of copper (II)
sulfate.
The liquid dripping into the beaker from the funnel will be colourless or slightly yellowish from the soil.
Acidify it with hydrochloric acid and add 2% barium chloride solution.
A thick white precipitate of barium sulfate forms (sulfate test).

18.2.5 Salinity
Water quality, Salinity tests, "Scientrific" (commercial website)
The salinity is the total solids, "salts", in water after all carbonates have been converted to oxides, all bromide and iodide have been
replaced by chloride, and all organic matter has been oxidized.
So it is a measure of the total dissolved salts in a water sample, mainly Ca, Mg, Na, bicarbonate, Cl and sulfate.

18.2.6 Conductivity, TDS and electrical conductivity
Environment meters, Conductivity, TDS, "Scientrific" (commercial website)
See diagram 18.2.6: EC and TDS
See 6.3.1.3: Distilled water
1. Electrical conductivity, (EC) measures the capacity of water to conduct electrical current, so is directly related to the concentration
of salts dissolved in water, Total Dissolved Solids (TDS).
Salts dissolve into positively charged ions and negatively charged ions, which conduct electricity.
Portable meters are used in the field to measure electrical conductivity where it is difficult to measure TDS.
Distilled water does not contain dissolved salts to conduct electricity, so has an electrical conductivity of zero.
However, at high salt concentrations electrical conductivity is not directly related to salts concentration.
TDS (ppm) = 0.64 × EC (μS / cm), (microSiemens / cm)

2. The total dissolved solids in water can be measured by evaporating a water sample but it is more convenient to use methods based
on water conductivity.
The conductivity is a measure of how well a water sample transmits an electric current.
It depends on the ionized substances dissolved in the water and temperature.
Conductivity is expressed in umhos per cm, and is usually measured across one centimetre.
Deionized water is a poor conductor of electricity with conductivity 0.5 to 2 umhos / cm.
However, water containing dissolved salts shows greater conductivity.
Usually conductivity is directly proportional to the concentration of dissolved salts in the water.
The conductivity of potable waters may range from 50 to 1500 umhos / cm.
Dissolved ionic matter can be estimated from conductivity by multiplying by 0.54 to 0.96, depending on components in water and
temperature.
Total dissolved solids, TDS, is the concentration of minerals and salts impurities in the water, measured in parts per million, ppm.
(1 ppm = 1 mg per litre).
Specific conductivity is expressed as mhos per centimetre (M / cm) i.e. siemens per centimetre, S / cm.
However, the mho (siemen) is a large unit, so usually the millimhos (millisiemen) (mS / cm) is used.
A value of 0.67 is commonly used to convert conductivity as mS / cm into total dissolved solids as ppm.
TDS ppm = Conductivity 0.67 S / cm × 0.67.
Electronic conductivity instruments can automatically compensate for temperature and correct readings to 25oC.
Many authorities think that potable water should contain less than 500 ppm of dissolved solids.

18.2.6.1 Tests for electrical conductivity with a conductivity meter
pH meters, conductivity meter, "Scientrific, (commercial website)
Remove the conductivity meter protective cap.
Stir the sample with the sensor tip for a few seconds, until the readings stabilize.
Record value as micromhos per centimetre.
Estimate total dissolved solids by multiplying the conductivity by 0.67.
Rinse the sensor tip with deionized water.
(conductivity: mho (ohm backwards) = 1 siemens (s) = ohm -1 = amps / volts)

18.2.7 Cations and anions in rain, rivers and sea water
Average chemical composition of natural waters, quoted by J. N. Butler, Carbon Dioxide Equilibria and Their Application,
Addison-Wesley, Reading, MA, 1982, Chapter 5
Table 18.2.7 Cations and anions in rain, rivers and sea water
Ion
Rain
Rivers
Sea water
Ca2+
.
0.53
10.6
Mg2+
.
0.21
54.6
Na+
0.009
0.39
479.0
K+
.
0.036
10.2
H+
0.072
.
.
NH4+
0.016
.
.
HCO3-
.
1.1
2.3
SO42-
0.028
0.21
28.9
Cl-
0.012
0.23
546.0
Br-
.
.
0.85
F-
.
0.008
0.07
NO3-
0.026
0.017
0.0001
H2SiO42-
.
0.15
.

18.3.0a Tests for air and dissolved oxygen in water
A good indicator of the health of water is how much air is dissolved.
Low air levels usually mean high levels of water pollution.
The mass of air that dissolves in water depends on the temperature of the water.
When water is heated to near boiling point, the dissolved gases become less soluble.

18.3.1 Tests for air dissolved in a water sample
Stand a beaker of water in sunlight.
Bubbles of air appear.
The taste of boiled water is different from tap water because boiled water has lost its dissolved oxygen.
Note the temperature of a sample of water.
Boil the water until no more bubbles appear.
Collect the air from the water in an inverted measuring cylinder.

18.3.2 Oxygen content of water, dissolved oxygen, DO, (Winkler method)
18.3.3 Tests for dissolved oxygen, titration
1. The oxygen content of pure water is usually 7 to 10 mg per litre, depending on the temperature and atmospheric pressure.
The following table shows the effect of temperature, at a constant pressure of 1013 mb (millibar) 760 torr.
(1 mm mercury 133.3 Nm-2)
The solubility of dissolved oxygen decreases as salinity increases.
Table 18.3.2 Dissolved oxygen, DO
Temperature oC Oxygen saturation mg / L
0 14.16
5 12.37
10 10.92
15 09.76
20 08.84
25 08.11
30 07.53
35 07.04
40 06.59

2. In natural stretches of water, the oxygen content is also affected by oxygen consumption because of contamination and the
breakdown process associated with it, and the production of oxygen because of the assimilation of underwater plants.
The oxygen wasting processes predominate, i.e. if the loading because of insufficiently purified, effluent, for example, is too great, the
stretch of water will gradually become a stinking, repulsive sewer in which life is no longer possible.

3. A sufficiently accurate test for determining the oxygen content of a water sample in schools is possible from the oxygen
determination method devised by L. W. Winkler.
This method uses the fact that when a manganous salt solution (e.g. manganous sulfate) is treated with caustic soda, a white precipitate
of manganous hydroxide is produced.
MnSO4 + 2 NaOH ---> Mn(OH)2 + Na2SO4
In the presence of oxygen, the manganous hydroxide is oxidized to brown hydrated manganese oxide.
2Mn(OH)2 + O2 ---> 2MnO(OH)2
The formation of hydrated manganese oxide is proportional to the amount of oxygen so that the intensity of the brown coloration is an
indication of the oxygen content.
The precipitate produced by the addition of manganous sulfate solution and caustic soda solution is coloured almost brown depending
on the oxygen content of the water sample.
The oxygen content may be estimated from the coloration.
If the precipitate remains white or almost white, there is no oxygen or very little oxygen present.
If the precipitate is light yellow, the water sample contains little oxygen.
If the precipitate is coloured brown, it is rich in oxygen.

18.3.3 Tests for dissolved oxygen, titration
Manganese ions react with potassium iodide to produce iodine.
Titrate the iodine with sodium thiosulfate using starch as an indicator.
Lower a sampler into the water from a convenient place, e.g. a bridge. Collect a water samples 1 metre below the surface by pulling a
string attached to the stopper of the sampler.
When bubbles no longer rise to the surface, note the water temperature and pull up the sampler.
Check the sample for bubbles that can give a false, high reading.
To the sample add 8 drops of manganous sulfate solution and 8 parts of alkaline potassium iodide azide. A precipitate forms.
Add 8 parts of 1 to 1 sulfuric acid.
Shake the sample until the reagent and the precipitate dissolve.
The colour of the sample is now clear yellow if dissolved oxygen is low to brown-orange if dissolved oxygen is high.
Add 8 parts of the starch indicator solution to the sample. It turns blue.
Titrate the sample against known molarity sodium thiosulfate solution until the blue colour becomes colourless throughout the water
sample.
Record the results as ppm dissolved oxygen.

18.4.0 Tests for ions in a water sample
See 12.11.3.2: Tests for metals with flame tests, metals and their compounds
Use the following test solutions:
Table 18.4.0 Test solutions
For Cl- AgNO3 solution turns milky white
For SO42- BaCl2 solution turns milky white
For Pb2+ Na2S solution forms a black precipitate
Evaporate a water sample in a non-aluminium container.
Heat slowly to avoid "spitting" when little water remains.
Dissolve the crystalline mass after evaporation in 10 mL of deionized water.
Add one drop of test solution and record the results.

18.4.1 Tests for phosphate ions in water
Excess phosphate ions in water can cause eutrophication.
Most modern detergents do not contain phosphate ions.
To check this, do the following experiment with 1 g of detergent dissolved in 1 g of water.
Dissolve 1.5 g di-sodium hydrogen phosphate (Na2HPO4.12H20) in deionized water and make up to 1 litre.
Make solutions with nine different concentrations by making up to 100 mL with deionized water the following volumes and label the
containers with the concentrations.
Be careful! Use a burette or a pipette with rubber suction attachment.
Do not suck by mouth!
Add 15 g of ammonium molybdate (VI)-4-water ([NH4]2MoO4] to 150 mL deionized water in a flask in crushed ice.
Leave the solution to cool.
Add 250 mL concentrated sulfuric acid to 250 mL deionized water.
Stop adding the acid when the flask becomes too hot, then leave it to cool in ice.
Slowly add the cold ammonium molybdate solution to the cold sulfuric acid solution.
Add 10 mL of ammonium molybdate and acid solution to each of the nine phosphate solutions.
Add 10 mL of ammonium molybdate and acid
solution to a 100 mL sample of water.
Add crystals of L-ascorbic acid and boil.
Compare the colour with the colour of the standard solutions.
This technique is called colorimetric analysis.
Table 18.4.1 Phosphate ions in water
L phosphate (20 ug / L) 20 mL phosphate (4 ug / L)
75 mL phosphate (15 ug / L) 10 mL phosphate (2 ug / L)
50 mL phosphate (10 ug / L) 5 mL phosphate (1 ug / L)
40 mL phosphate (8 ug / L) 0 mL phosphate (0 ug / L)
30 mL phosphate (6 ug / L) .

18.5.1 Tests for characteristics of river, lake or ocean
1. Sample number, names of testers
2. Location: Distance from shore and location upstream or downstream from a marker
3. Date and time
4. Weather: Fine or cloudy or rainy, wind speed and direction
5. Air temperature, daily range, oC
6. BOD, Biochemical Oxygen Demand, mg / L
7. Colour and appearance.
Compare the colour of the water against a white background.
Appearance: scum, muddy, clear, brown, foamy, milky.
8. Current flow and depth: stagnant, calm, brisk, raging.
9. Detergents: Half fill a flask with river water, insert a stopper and shake for one minute, rate as "no detergent" if bubbles disappear
in less than three seconds, rate as "slightly frothy" if the bubbles take up to ten seconds to break up, rate as "frothy" if the froth takes
up to five minutes to disperse.
10. Dissolved oxygen, ppm, % saturation
11. Faecal coliform bacteria colonies / 100 mL
12. Microscopic examination: Note the size of suspended particles and any life forms, e.g. algae.
13. Oil or petrol: Look for the rainbow effect of petrol or oil on water.
14 pH
15. Smell: no distinctive character, musty, fresh, putrescence, earthy, sewage-like, putrid, like liquid manure, peaty, chemical.
16.0 Turbidity
17. Tolal nitrates, mg / L
18. Total phosphorus, mg / L
19.0 Total solids
20. Visibility: Use a Secchi disc at depth of one metre
21. Water temperature, daily range, oC
Each item can be given a comparative rating so that an overall rating can be calculated for comparison with other sites.

16.0 Tests for turbidity
See: Water quality, Turbidity sensor, Secchi Disk, "Scientrific", (commercial website)
Turbidity, mg / L.
Observe settling on standing after 30 minutes.
Precipitate any fine suspensions and colloids that pass through the filter paper by adding aluminium potassium sulfate (potassium alum,
Al2(SO4)3.K2(SO4).24H2O) then filter.
However, you can also use device called a nephelometer to measure turbidity, NTU.

19.0 Total solids
See: Water quality, TDS, Total dissolved solids, "Scientrific", (commercial website)
Total solids, mg / L, Conductivity, umhos / cm, conductivity × 0.67 = TDS, total dissolved solids, Floating debris

18.5.1a Eutrophication of waterways
Waterways can be have excess plant nutrients from leaching of land, especially agricultural land containing excess fertilizers, and
discharge of effluents containing nitrogen and phosphorus.
Decay of water weeds and other primary organic matter causes depletion of oxygen especially in the summer, e.g. decay of
Eichhornia crassipes
, water hyacinth, "the worst weed in the world", South America, Pontederiaceae.

18.5.2 Test for anions in sewage and tap water
See 12.11.5.0: Tests for anions in unknown solution, tests for acid radicals in solution
Compare concentrations by comparing the intensity of colour or amount of precipitate.

18.5.3 Tests for water samples
A list of tests used by some senior chemistry classes
1. Salinity: meter and refractometer
2. Turbidity: Tube and meter
3. Nitrite: Hach test strips
4. Nitrate: Hach test strips and spectrophotometer (OxidN in guidelines)
5. Phosphate: Hach test strips and spectrophotometer (convert to FiltRP in guidlines)
6. pH: Narrow range Neutralit pH strips and meter
7. Dissolved oxygen: Meter and spectrophotomter
8. Conductivity: Meter

Many individual tests are necessary to detect with certainty the purity or contamination of a stretch of water, including
physico-chemical, bacteriological and biological test methods.
You can study water samples taken from a river before and after passing through a community area.
The physico-chemical examination of the water samples can include tests for the presence of pollutant indicators besides determining
the smell, colour, temperature, and oxygen content.
These pollution indicators are substances because of contamination with faecal matter in the water.
However, many of these compounds are normally present in water in small amounts so precise guidelines and maximum permitted
values have been decided.
Occasionally, however, these values may differ considerably from the specified figure, because of geological conditions for example,
although no contamination of a type that is harmful to health may be present in the water.
The results of the individual physico-chemical tests must therefore only be used as a whole for assessing the quality of water.
A selection of the most important physico-chemical tests for judging the purity or contamination of a stretch of water, which can also
be done without great expense using facilities available in schools is given below.

18.6.0.1 Commonly occurring air pollutants, safe air and clean air
In new estimates released on 25 March, WHO reports that in 2012 around 7 million people died, (1 in 8 of total global deaths), as a
result of air pollution exposure.
This finding more than doubles previous estimates and confirms that air pollution is now the world's largest single environmental health
risk.
The new data reveal a stronger link between both indoor and outdoor air pollution exposure and cardiovascular diseases, such as
strokes and ischaemic heart disease, as well as between air pollution and cancer.
Much of the burden results from burning of fossil fuels, and the new figures underline the opportunity for more sustainable development
choices to simultaneously save lives and mitigate climate change.
Table 18.6.0.1 Air pollutantszzz
Pollutant
Source
Health
Safe air
Clean air
sulfur
dioxide
sulfur in fuel
lung disease
2 pphm
(annual mean)
0.01
ppm
nitrogen
oxides
high temperature
combustion
lung disease
16 pphm2
(1 hour average)
< 0.01
ppm
dust,
soot
combustion, mining,
land clearing
lung disease
if acid gases
90 mg / m3 TSP,
(annual mean)
10-20
g / m3
ozone
nitrogen oxides
and carbon
lung irritation
12 pphm
(1 hour average)
nil
lead
smelting,
leaded petrol
cumulative
poison
1.5 mg / m3
(3 month mean)
nil
carbon
monoxide
motor vehicle
combustion
body motor
functions
9 ppm2
(8 hour average)
< 1
ppm
mg = micrograms, pphm = parts per hundred million, ppm = parts per million, TSP = total suspendable particulate

18.6.0.2 Indoor air pollution, formaldehyde pollution
The pollution in an indoor environment includes: suspended particulates, smoke nuisance, volatile and semi-volatile organic
compounds, formaldehyde, and burning gases.
Formaldehyde pollution is caused by 1. pressed wood products (hardwood plywood wall panelling, particleboard, fibreboard) and
furniture made with these pressed wood products, 2. urea-formaldehyde foam insulation (UFFI), 3. combustion sources and
environmental tobacco smoke, 4. durable press drapes and other textiles, 5. glues.
Formaldehyde gas can cause watery eyes, burning sensations in the eyes and throat, nausea, and difficulty in breathing in some humans
exposed at elevated levels (above 0.1 parts per million).
Health effects include eye, nose, and throat irritation; wheezing and coughing; fatigue; skin rash; severe allergic reactions.
Average concentrations in older homes are usually well below 0.1 ppm.
In homes with many new pressed wood products, levels can be greater than 0.3 ppm.

18.6.1 Tests for contamination of groundwater by refuse, hazardous wastes
The main problem of waste disposal is the possible contamination of groundwater by direct seepage from refuse deposits or by
substances, leached from such deposits.

1. A 100 mm funnel is supported from a support stand using a right angle clamp and a universal clamp.
Put a small wad of cotton wool in the funnel and fill it with soil to within a thumb width of the top.
Put a beaker beneath. Spread l g copper (II) sulfate crystals on the soil.
Pour water is poured over it.
The water dissolves the copper (II) sulfate and seeps into the soil.
After a few minutes the blue copper (II) sulfate solution drips from the funnel into the beaker beneath.

2. Spread an equal amount of sodium sulfate, potassium sulfate or ammonium sulfate on the soil in the funnel, instead of copper (II)
sulfate.
The liquid dripping into the beaker from the funnel will be colourless or at any rate only slightly yellowish from the soil.
On acidifying it with hydrochloric acid and adding some 2% barium chloride solution, a thick white precipitate of barium sulfate is forms.

3. Hazardous wastes must be disposed on in a geologically responsible way, usually by incineration in special furnaces.
Hazardous wastes include pesticides, car batteries, petrol, oil, swimming pool chemicals, solvents, aerosol products, fire extinguishers,
barbecue gas bottles, paint fluorescent lamps and tubes.
Ask your local city council or town council how hazardous wastes should be disposed of and what facilities for disposal are available
to individual householders.

18.6.2 Tests for air pollution from burning refuse
Considerable pollution of the environment by harmful combustion products can be caused by the burning of rubbish that is often done
without proper understanding.
A typical example is the burning of polyvinyl chloride (PVC). This synthetic plastic is used to make many types of domestic objects,
but it is also used as a packaging material that is later scrapped becoming refuse.
When it is burnt, the chlorine combined in its molecules is converted to hydrogen chloride among other products and this dissolves in
the water produced to form dilute hydrochloric acid.
The World Health Organization advises that the acceptable level of fine particles in the air measuring less than 2.5 microns, known as PM2.5,
should be no more than 25 micrograms per cubic metre.
Above 300, and the United States Environmental Protection Agency warns that outdoor activity becomes hazardous, even for healthy adults.
On January 12, 2013 in Beijing, the PM2.5 pollution hit 886, officially off the charts for dangerous air quality.
The problem is not limited to Beijing.
Many other cities in China have also been suffering from what is described as the worst air pollution in history.

Experiments
18.6.2.1 Tests for air pollution from burning refuse
1. Metaldehyde
A few small PVC rods are laid on two small tablets of solid fuel (Esbit, Blitzem, firelighter, "meta" fuel, canned heat, snail bait,
metaldehyde, in a porcelain basin.
A strip of moistened, blue litmus paper is hung 25 cm above the basin, using a support rod, a right angle clamp and a universal clamp.
The fuel is set alight and the PVC is burnt.
Within no more than a minute, the blue litmus paper is turned red by the hydrochloric acid formed by the burning PVC.
The experiment should be done in a fume cupboard.
Prove that the red coloration of the blue litmus paper is because of combustion of the PVC, two tablets of solid fuel without any PVC
are burnt in a parallel experiment.
The colour of the blue litmus paper is unchanged even after the two tablets have completely burnt away.

2. Put a porcelain dish containing a tablet of solid fuel and PVC rods on a glass plate.
The solid fuel tablet is easier to ignite when it is broken in two and one piece is placed at an angle across the other.
Use a 5 litre glass bell jar with a strip of moistened blue litmus paper secured by a rubber stopper hanging 10 cm inside the neck.
Invert the glass bell jar over the porcelain dish when the solid fuel has been ignited.
Although only a little PVC burns in the small quantity of air enclosed under the bell jar, the colour of the litmus paper turns strongly to
red.
These experiments show that rubbish should be deposited only on sites where the nature of the ground makes it unlikely that
groundwater can become polluted by substances leached from the rubbish.
Burning of the rubbish to reduce its bulk should be permitted only in appropriately designed refuse destructors in which the resulting
combustion products can be rendered
harmless.

18.6.3 Danger of vehicle exhausts, tailpipe gases
Exhaust gases from motor vehicles contain lead dust (5 to 30 mg / m3), nitric oxide (0.005 to 0.3% by volume), hydrocarbons
(0.01-1%), and carbon monoxide (1-10% by volume).
Carbon monoxide is especially dangerous to human beings because it cannot be detected by the senses because it has no colour,
smell or taste.
Its affinity to haemoglobin is 250 times that of oxygen.
During carbon monoxide poisoning, a rapid breakdown in the supply of oxygen to the body occurs, leading to headaches and
dizziness at low concentrations.
At high concentrations (0.2% by volume) it can very rapidly lead to death.
To monitor the carbon monoxide content of the air you can use a carbon monoxide gas detector, e.g. the gas detector, that
allows detection of concentrations as low as 0.001% by volume.

Experiments
18.6.3.1 Tests for carbon monoxide with a gas detector
Make ten strokes of the pump and squeeze the bellows of the pump until they reach their limit so that the suction stroke admits
100 mL of air.
The colour of the preparation in the test-tube changes while the pumping is in
progress.
The white indicator layer, which contains iodine pentoxide (I2O5) as the effective reagent with fuming sulfuric acid (H2S2O7) turns
brown-green during the reaction time, under the influence of selenium dioxide (SeO2) as catalyst.
The reaction can be described by the following equation:
SeO2 + H2S2O7
5CO + I2O5 ---> I2 + 5CO2
The length of the coloured zone depends on the carbon monoxide concentration.
The carbon monoxide content of the air, in percentage volume can be read directly on the printed scale.
MWC, maximum working concentration, is that concentration in the air of a workshop, measured in depth of breathing, at which no
damage to health is to be expected, even with exposure during the day.
Comparative measurements of the carbon monoxide content of the air should be repeated under different weather conditions,
because the carbon monoxide content of the air depends on rain, mist, wind, sunshine and smog conditions with an inversion layer.
Table 18.6.3 Carbon monoxide effects
CO content of the
air in vol.
per cent
CO concentration
in ppm
CO concentration
in the blood
Effect on humans
0.01 100 (MWC) 10 to 20% no perceptible effect
0.025 250 30% headaches, slight fatigue
0.05 500 40 to 50% headaches, collapse and
fainting on exertion
0.1 1000 60 to 70% unconsciousness,
cessation of breathing
on prolonged action
0.2 2000 >70% instant death
Nowadays, direct plug-in electrochemical 12-24V in-car carbon monoxide alarms are available to help detect dangerous levels of
carbon monoxide in motor vehicles and workshops.

18.6.4 Water tests
Use individual tests to find the degree of purity or contamination of water.
Tests include physicochemical, bacteriological and biological tests. You may do the tests at sites in a river before and after passing
through a community area.
Test for the presence of "pollutant indicators" besides determining the smell, colour, temperature, oxygen content.
These indicators are substances in the water because of contamination with faecal matter.
Many of these compounds are normally present in water in small amounts so precise guidelines and maximum permitted values have
been laid down.
These values may differ considerably from the specified figure, because of geological conditions, although no contamination of a type
that is harmful to health may be present in the water.
So the results of the individual physico-chemical tests must be considered as a whole for assessing the quality of water.
Some more important physico-chemical tests for judging the degree of purity or contamination of water are described below.

18.6.5 Smell of water, hydrogen sulfide
1. Water used for drinking must not smell unusual, repugnant, or revolting.
Many substances can affect the smell of water.
The bad smell of underground water may be caused by hydrogen sulfide that can be produced by the reduction of iron sulfide.
Make a preliminary test of smell to provide initial information when the sample is taken because some smells, like that of hydrogen
sulfide, may rapidly disappear.
You can more readily detect odours if the substance is heated slightly.
Hydrogen sulfide, when dissolved in water, produces an offensive odour resembling that of rotten eggs.
The presence of hydrogen sulfide in deep well water is because of the reduction of sulfate.
The acceptable level of hydrogen sulfide is 0.05 mg / L or less.
Hydrogen sulfide can be removed through oxidation or by aeration or chlorination.

2. The precipitated sulfur should be removed by filtration to prevent it from reverting back to hydrogen sulfide through the action of
certain micro-organisms.
The oxidation of hydrogen sulfide by chlorine may be advantageous in cases where it is otherwise unnecessary to repump the water,
normally required with aeration, because chlorine can be applied directly into the system.
Enough chlorine must be used to maintain a distinct chlorine residual.

Experiment
18.6.5.1 Tests for smell of water
Put 100 mL of the water sample in a 250 mL wide mouth bottle.
Close with a glass stopper and heat in a water bath to 40oC.
After shaking it vigorously, open the bottle and test the smell of the water immediately.
Unbiased measurement of smell by means of numerical values is impossible but use the following terms to describe the smell:
1. no distinctive character, 2. musty, 3. fresh, 4. putrescent, 5. earthy, 6. sewage-like, 7. putrid, 8. like liquid manure, 9. peaty,
10. chemical.
The general description "chemical" can be amplified, as smelling of hydrogen sulfide, chlorophenol (pharmacy shop smell), chlorine, tar,
ammonia, mineral oil, phenol.
Use the terms "slight or "pronounced" to describe the intensity of the smell.

18.6.6 Colour of water
1. Pure, clean water is colourless or possibly just slightly bluish in colour.
A colour other than this may be because of the most different kinds of foreign or contaminating matter.
Thus humus materials generally produce a yellow brown coloration, iron a yellowish reddish one, micro-organisms, e.g. plankton
organisms may give a greenish, yellowish or brownish colour to the water.
Water that is distinctly contaminated has a greyish yellowish to grey black colour.
Different substances that may cause differing amounts of harm may produce similar discoloration so an unbiased measurement using
numerical values is impossible.
It follows that the colour of water used for assessing its quality must be used only with all the other test results.
Devices called tintometers can be used to record water colour.
The name of a colour may be based on the "Munsell colour chart".

Experiment
18.6.6.1 Tests for colour of water
2. Use two tall colourless glass beakers.
Put 200 mL of the water sample in one beaker.
Put the same amount of deionized water in the other beaker. Put both beakers on a white background, e.g. a sheet of writing paper
or a white tile.
The colour of the water sample, as viewed from above, is compared with that of the distilled water.
It can be described as follows: colourless brown, slightly yellowish, yellowish green, yellowish, greenish, yellow, green, yellow brown,
grey yellow, brownish, grey black.
The room must be well-lighted by daylight.

18.6.7 Hydrogen ion concentration of water
1. Pure water is very slightly dissociated, i.e. split into its ions (H+ and OH-).
One litre of pure water contains 1 / 10 000 000 (10-7) g of hydrogen ions (H+).
Since the same amount of hydroxyl ions (OH-) is also present, pure water has a neutral reaction.
For the sake of simplicity, instead of the value 10-7, the pH value is used, which is the logarithm of the reciprocal of the hydrogen ion
concentration.
Water with a pH value of 7 is neutral; below it is acid and above 7 it is alkaline.
Natural stretches of water usually have a pH value approximating to that of the neutral point.
Extreme values, for example because of the nature of the soil from which the water originates, are pH 3 in the acid range and pH 12
in the alkaline range.
The organisms living in water thrive best of all at a pH between 6.8 and 7.8.
Changes of hydrogen ion concentration, e.g. because of the introduction of insufficiently neutralized industrial effluent, may cause gross
disturbances in the ecological equilibrium.
Also, the changes may cause the direct poisoning of underwater life because of the materials introduced.

Experiment
18.6.7.1 Tests for hydrogen ion concentration of water
The simplest way to find the pH value is to use universal indicator paper, pH 1 to 10, for the whole pH range and as special
indicator paper for various pH ranges, e.g. indicator rods: pH 2.5 to 4.5, pH 4.0-7.0, pH 6.5 to 10.0.
First wet a strip of universal indicator paper the water sample.
After one minute find the pH value by comparing the colour produced with that of the colour scale provided.
The colour scale of the universal indicator paper is subdivided into complete pH units.
By estimating the intermediate stages, half pH units can also be read off.
The test is repeated in the same way, using a strip of special indicator paper of the corresponding pH range.
The graduation of the colour scale of the special indicator paper is so fine that 2 / 10 of a pH unit can be read off.
By this means, determining the hydrogen ion concentration of a water sample with an accuracy normally sufficient for school purposes
is possible.
For more accurate measurements use a battery-operated electronic pH meter.

18.6.8 Temperature of water
1. Drinking water should be neither too hot nor too cold and have a temperature between 8oC and 12oC.
Colder water is generally regarded as unacceptable.
At temperatures below 5oC, stomach or intestinal troubles may even occur.
Water at a temperature of more than 15oC has no longer a refreshing effect.
The temperature is important for the ecological equilibrium of a stretch of water since the oxygen content of water is very closely
related to it.
The introduction of large amounts of insufficiently cooled cooling
water into a river by an industrial undertaking may have catastrophic consequences and, e.g. contribute to the mass death of fish
observed recently.

Experiment
18.6.8.1 Tests for temperature of water
Tie a rope, e.g. a Perlon cord 0.5 mm in diameter, just above the spherical bulb at the lower end of an aquarium thermometer.
Also, a piece of metal, e.g. an old key, is attached to make it sink in water.
Tie pieces of red string at intervals of 25 m.
This device allows temperatures to be taken at various depths in water.
The thermometer is let down, the depth of water is recorded, and after two minutes it is pulled up quickly and the temperature
immediately read.
Temperature measurements on water samples are done in the workroom using a chemical thermometer.

18.6.9 Colorimeter tests, test methods
Water quality, Colorimeters, "Scientrific", (commercial website) Tests for to Test method
Aluminium to Eriochrome, Cyanine R
Ammonia to Nitrogen, Salicylate
Ammonia to Nitrogen, Nesslerization,
Bromine to DPD, Tablets
Chlorine to DPI, Tablets
Iodine to DPI, Tablets
Chlorine Dioxide to DPI, Tablet / Glycine
Chromium (Hexavalent) to Diphenylcarbazide
Chromium (Total, Hexa and Trivalent) to Diphenylcarbazide
COD, Low to Digestion (mercury free)
COD, Low to Digestion contains mercury
COD, Standard to Digestion (mercury free)
COD, Standard to Digestion (contains mercury)
COD, High to Digestion (contains mercury)
Copper to Diethyldithiocarbamate
Copper to Bicinchoninic Acid
Cyanide to Pyridine to Barbituric Acid
Cyanuric Acid to Melamine
Fluoride to SPADNS
Hydrazine to P to dimethylaminobenzaldehyde
Hydrogen Peroxide to DPI Tablets
Iron to Bipyridyl
Iron to 1,10 Phenanthroline
Manganese (LR) to PAN
Manganese (HR) to Periodate
Molybdenum to Thioglycolate
Nickel to Dimethy1glyoxime
Nitrate Nitrogen to Cadmium Reduction
Nitrite Nitrogen to Diazotization / Coupling
Oxygen, Dissolved to Winkler Colorimetric
Ozone to Indigo Trisulfonate
pH (5.0 to 9.6) to Colorimetric
Phenols 4 to Aminoantipyrine
Phosphate (HR) to Molybdovanadate
Phosphate (LR) to Ascorbic Acid Reduction
Potassium to Tetraphenylboron
Silica (LR) to Heteropoly Blue
Silica (HR) to Silicomolybdate
Sulfate to Barium Chloride
Sulfide to Methylene Blue
Tannin to Tungsto to Molybdophosphoric Acid
Turbidity (0 to 400 FTU) to Absorptimetric
Zinc to Zincon

18.7.0 Bottled Water
For many years, we relied exclusively on rainwater or tap water for drinking.
Now that consumers are turning to bottled water as the healthy and "pure" alternative to tap water, are we really just wasting our
money and the environment? Spring water is extracted from natural sources.
Other bottled water is simply water from the municipal water supply that has been subjected to additional treatment.
Consumers may claim that bottled water is convenient and that it tastes better than tap water.
It is also marketed as the healthy alternative to sugared drinks and as the most "pure" available water.
But the health arguments for bottled water are overstated.
The choice is not one between sugared drinks and bottled water, but between bottled water and tap water.
There is no evidence that bottled water is more "pure" than tap water, but plenty of evidence to suggest that bottled water is costly:
both to the hip pocket and to the environment.
During extraction spring water is extracted from underground aquifers upstream from where the water surfaces.
This disrupts aquifer flow, affecting flora and fauna.
In production, most bottled water is packaged in PET (polyethylene terephthalate) plastic bottles, which are derived from crude oil.
It can take up to 3L of water to produce 1L of water.
Transportation of bottled water around the world requires burning of fossil fuels.
In landfill and the litter stream, although plastic bottles are recyclable, many end up in landfill and take up to 1000 years to break down.
When littered they often end up in the sea where they break up in small pieces, killing marine life that mistake them for food.
The best thing to do is to avoid bottled water.
Australian tap water is world standard drinking water so it is the safe, cheap and sustainable option.
Install a tap filter if you are concerned about the taste or quality of your local tap water.
Buy a reusable bottle.
There is a great range available from camping stores and other retailers.

Using rivers for water testing
There are two experiment methods:
1. Manipulate one variable to see the effect on the other, keeping other variables constant, e.g. What is the effect of temperature
(the manipulated or independent variable) on the concentration of Vitamin C in orange juice, (the dependent variable)?
2. Concomitant variation.
A naturally occurring variation (Variable 1) is correlated against another variation (Variable 2).
The correlation method.
For example: How does temperature in the natural environment affect stoma opening?
In this experiment you do not need you to control the environmental temperature, but you do need to measure the dependent variable
at different temperatures.
However, there may be other potentially influential variables, e.g. humidity.
That does not preclude the variables in relationship being considered.
One way to address the other variables is to collect data on the other variable as well and run the statistics on each pair of variables
separately.
Where the data are collected from a local river, the method used is the second approach.
Students are relying on some naturally occurring change in water quality, e.g. BOD, turbidity, temperature as one set of variables,
then trying to correlate this with the riverside development at sites along the river.
However, there are so many confounding variables and the differences between two sites may be many).
A river may be just used as a source of field data for collecting samples for water quality testing.

18.7.1 Drinking water test media
Bismuth Sulfide Agar, Brilliant Green Agar, Brilliant Green Bile Lactose Broth, Cetrimide Agar, DCLS Agar, ENDO Agar (Base),
Formate Ricinoleate Broth, MacConkey Agar No 1, Malachite Green Broth, Methyl Red Voges Proskauer Broth, Nutrient Broth No 3,
Peptone Water, Phenylalanine Agar, Plate Count Agar, Selenite Broth (Base), Simmons Citrate Agar, SS-Agar, Tetrathionate Broth,
TetrathionateBroth (Base), Triple Sugar Iron Agar, Tryptic Soy Broth ,Tryptone Glucose Extract Agar, Tryptone water, Urea Broth,
Violet Red Bile Agar

18.7.2 Effect of household detergents on freshwater organisms
Most detergents are "biodegradable", but degradation takes time, so detergents in rivers and lakes may be harmful before they are
degraded.
Detergents may affect the permeability of biological membranes.
Investigate the tolerance of certain freshwater organisms to different concentrations of detergents.
Use differernt organisms for testing, e.g. Daphnia, freshwater snails, small fish, the monocotyledon duckweed Lemna, and tadpoles.