Status message

Submissions for this form are closed.

Toolbox for Protein Characterisation

UQ Research Facilities are collaborating to share their latest technologies in protein characterisation with UQ researchers. At this workshop, you will learn about the different characterisation tools available at UQ for:

  • protein identification and mass determination
  • thermal stability measurement
  • buffer formulation to prevent protein aggregation and improve protein stability
  • protein complexes imaging
  • quantitative measurement of particle size distribution

This is a fantastic opportunity to meet and speak with experts in this field and learn where you can access this state-of-the art equipment.

Workshop speakers include:

Dr Amanda Nouwens, Facility Manager, SCMB Mass Spectrometry Facility

Protein Analysis by Mass Spectrometry in SCMB

Mass spectrometry is a fabulous tool for analysis and characterisation of proteins.  Possibilities include identification of proteins (pure and complex mixes), intact mass analyses for accurate molecular weight, quantification, characterisation of post-translational modifications, and even native mass spectrometry to look at protein-protein and protein-ligand interactions.  Examples from real projects utilising the SCMB MS facility will be presented, with sample prep requirements and practicalities discussed.

Christopher Munro, Downstream Processing Specialist, UQ Protein Expression Facility

Characterising Protein Stability: An Integrated Approach

Characterising protein stability is a crucial step to prevent protein aggregation, which can lead to loss of protein biological activity. Successful characterisation relies on the availability of specialised equipment capable of detecting aggregates and measuring subtle changes in protein stability. This presentation will highlight factors influencing protein stability and demonstrate the need to incorporate multiple analytical tools in order to achieve high quality, stable recombinant proteins.

Trent Warburton, Product Manager, TrendBio Pty Ltd

NanoDSF: a new technology to analyse protein stability

nanoDSF is an advanced Differential Scanning Fluorimetry developed by NanoTemper, which measures ultra-high resolution protein stability using intrinsic tryptophan or tyrosine fluorescence. Changes in the fluorescence will allow for the chemical and thermal stability of the protein to be assessed in a truly label-free approach. The dual-UV technology by NanoTemper allows on-the-fly fluorescence detection and temperature induced protein aggregation is measured simultaneously with NanoTemper’s back reflection technology. Concepts and applications of these technologies will be highlighted and presented.

Professor Roger Wepf, Director, Centre for Microscopy and Microanalysis

Rick Webb, Senior Laboratory Manager, Centre for Microscopy and Microanalysis 

Protein Characterisation by Electron Microscopy at CMM: from quality control to atomic reconstruction

Protein folding and their final structure (secondary, tertiary and quaternary structure) are essential for their functionality and specific function. Hence the 3D structure of proteins - resolved by X-ray crystallography and single particle Electron Microscopy (EM) finally complemented by NMR dynamic structure determination - is of interest to elucidate the structure-function relation of any isolated or synthesised protein or protein–protein complexes. We will briefly introduce these EM based methods to give attendees a better understanding of pros and cons of these methods and their prerequisites as well as the needed effort and resources. 

Who should attend?

Postdoctoral researchers, research staff and Research Higher Degree students are highly encouraged to attend.

Workshop Details:

Date: Tuesday 23rd May 2017

Time: 8:45am - 12:30pm (Registration starts at 8:45am, for a 9am start)

Venue: AIBN Seminar Room, Level 1

Light refreshments will be provided for all registered participants

Bookings close: Friday 19 May or when full

 

We welcome you to our free and informative protein characterisation workshop. Please enter your details below and click submit to register.

Please contact Chris on: c.steel1@uq.edu.au if you have any probems with your registration. Bookings close: Friday 19 May or when full.