The Leishmania tarentolae extract (LTE) in vitro translation system is a rapid, convenient, flexible and cost effective tool to produce recombinant proteins for biochemical, biophysical and structural analysis (Mureev et al., 2009, Kovtun et al., 2010).

pLTE Products and Services offered through PEF:

 

Product/Service
Description
Available from

Cell-free Protein Expression Kit (Plasmid Based)
- 70 reactions

Translational competent LTE cell extract
- LTE cell extract (1ml)
pLTE expression vector
- 9 µg

Please enquire

Cell-free Protein Expression Kit (PCR Based)
- 70 reactions

Translational competent LTE cell extract
- LTE cell extract (1ml)
Species independent translation sequence (SITS) fragment
- 600 ng
Forward primer for overlap extension PCR
- 50 µl of 10 µM
pLTE (EGFP control) vector
- 9 µg

Please enquire

pLTE vectors

PEF offers 5 different vectors:
pLTE (EGFP control)
pLTE_6H_EGFP_3C
pLTE_3C_EGFP_6H
pLTE_6H_N and
pLTE_6H_C

More info on vectors...

Please enquire

Plasmid-based Cloning with Optional Cell-free Expression

Order cloning and small-scale expression testing via the PEF Resource Centre.
Cell-free vectors available:
pLTE_6H_EGFP_3C
pLTE_3C_EGFP_6H
pLTE_6H_N and
pLTE_6H_C

More info on vectors...

Resource Centre

PCR-based Cloning with Expression Screening

Order cloning with Expression Screening via the Template Design Shop.

Resource Centre

Related Manuals:

Plasmid based LTE Cloning manual (pdf)

PCR based LTE Cloning manual (pdf)