QBP Cryo-TEM Facility

Located in the Queensland Bioscience Precinct at The University of Queensland, the Cryo-Transmission Electron Microscopy (TEM) Facility is a laboratory that was purpose-built for standard and cryo-TEM sample preparation and analysis, as well as electron tomography of both resin-embedded and cryo-samples (for three-dimensional analysis) 

 
The facility is equipped with three TEM's and one scanning electron microsocpe (SEM):
- Tecnai F30 300keV Field Emission Gun (FEG) TEM with a 4k Direct Electron LC1100 (lens-coupled) Camera
- Tecnai T12 120keV TEM, with a 4k FEI Eagle CCD Camera  
- JEOL 1011 100 keV  TEM with an Olympus SIS Morada CCD Camera
- JEOL JCM5000 Benchtop SEM (Neoscope)
 
 
Ancillary equipment for sample preparation available are:  
- two high-pressure freezer systems: BalTec HPM010 and Leica EM PACT2
- Leica AFS and EM-AFS2 (automatic freeze substitution systems) with an automated freeze substitution processor
- PELCO Biowave microwave
- FEI Vitrobot
- Leica EM UC6 ultramicrotomes and cryo-ultramicrotomes
- Nikon Eclipse Ti-E fluorescence microscope
- Tousimis Auto Critical Point Dryer
 
 
The major techniques we offer are:
- High-pressure freezing of biological samples
- Plunge freezing of particle suspensions 
- Biological sample preparation (standard and microwave-accelerated), via freeze substitution or chemical fixation, and resin embedding 
- Ultramicrotomy and cryo-ultramicrotomy
- Standard and cryo-TEM analysis
- Electron tomography data acquisition and processing
- correlative light/electron microscopy

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