Event Details

Wednesday, 02 November 2016
12:00 pm - 1:00 pm
QBI Level 7 Auditorium
UQ Location:
Queensland Brain Institute (St Lucia)
Event category(s):

Event Contact

Ms Deirdre Wilson
334 66300
Org. Unit:
Queensland Brain Institute

Event Description

Full Description:
Natalie Lee,
Queensland Brain Institute, University of Queensland

Title: The role of Neogenin in epithelial morphogenesis

Abstract: Radial glial progenitors are neuroepithelial cells and give rise to all neurons in the embryonic brain. As for all epithelia, adherens junction (AJ) formation and maintenance in radial progenitors are dependent on cadherin-mediated cell-cell adhesion. Failure in AJ assembly leads to loss of apico-basal polarity, which destroys progenitor morphology and results in the failure to generate neurons. Previous studies in the Cooper lab revealed that depletion of the guidance receptor neogenin in the embryonic mouse cortex resulted in severe disruption of progenitor morphology due to a failure in AJ assembly. Moreover, loss of cell adhesion leads to the formation of neuronal heterotopias protruding into the ventricle and below the cortical plate.
We have now investigated the role of neogenin in AJ formation using the well-established in vitro epithelial CaCo2 cell model. We show that loss of neogenin disrupts cadherin homophilic adhesion between adjacent cells, leading to a severe disruption in junctional contacts. Furthermore, loss of neogenin also results in the inability to recruit key actin nucleating factors such as the wave regulatory complex and the actin polymerization complex Arp2/3 to the cell-cell interface. This inability leads to dramatic changes in junctional tension due to a significant decrease in the actin turnover rate at AJs. Together, these findings suggest neogenin may control neurogenesis in the developing cortex by regulating AJ assembly, actin nucleation, and actomyosin-mediated tension between radial glia progenitors.

Directions to UQ

Google Map:
To St Lucia Campus, UQ Ipswich, and UQ Gatton.

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